If glycoside is present yellow to orange colour will be seen. Take a piece of lamina or thick section of the leaf and add sodium picrate reagent. Different tests are done because of the different chemical entities of the aglycone moiety. Give positive reaction with molischs and fehlings solution test after hydrolysis. We have to hydrolyze the drug and then chemical tests are carried out. Glycosides chemical properties purvi kakrani, glycosides, lbriper 9 acid hydrolysis. Violet colors appears, this is due to presence of active methylene group. Coumaringlycosides chemical structure class sigmaaldrich. Glycosides classification and isolation tests part 2. Test for phenols a small amount of the ethanolic extract was taken with 1 ml of water in a test tube and 1 to 2 drops of iron iii chloride fecl 3 was added. A solution of glycosides is treated with a small amount of kedde reagent mix equal volumes of a 2% solution of 3, 5 dinitrobenzoic acid in menthol and a 7. Hcl for 10 min, filter and shake with organic solvent ether or benzene.
They are water soluble compounds, insoluble in organic solvents. A blue, green, red or purple color is a positive test. Usa home product directory biochemicals and reagents antibiotics chemical structure class coumarin glycosides life science home life science products. All glycosides are hydrolysable by acids except c glycosides. For c types of anthraquinone glycosides i modified brontragers test. Acetal linkage between the aglycon and glycone more unstable than that between two individual sugars within the molecule. Physical and chemical properties colourless or white crystals rarely amorphous solubility is determined by the number of sugar moieties. Pdf pharmacognosy chemical tests swathi chinthapatla. It is a semi quantitative method to find out the presence of sugars that is polysaccharides. A purple colour is presence of to an aqueous solution 20ml cuso4 slo 1 drop is formed isobarbaloin aloe added followed by nacl 1g and 90% alcohol 10ml. Test for glycosides a small amount of alcoholic extract was taken in 1 ml of water in a test tube and a few drops of aqueous naoh. Physicochemical properties of glycosides colorless, solid, amorphous, nonvolatile flavonoid yellow, anthraquinonered or orange. The procedure for the isolation of glycosides also varies. Pale yellow precipitate presence of to the sample add bromine.